sandbox-explanation
The sampling set-up is a rack of plastic tubes filled with sand, or "sandboxes." We used sand because we are interested in the very first stages of microbial succession when there are no nutrients or living things. While it hard to remove microorganisms and nutrients from potting soil, it is easy to sterilize and remove nutrients from sand. By removing nutrients, we are forcing our microbial colonists to either make everything they need to survive from scratch or carry it with them.